HelixCript™ One-Step RT-PCR Kit [Hot-Taq] 의 bulk version 입니다.

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HelixCript™ One-Step RT-PCR Kit [Hot-Taq] 는 주형 RNA로부터 역전사 반응(reverse transcription)과 PCR 반응을 하나의 tube 반응을 통해 target transcript를 증폭할 수 있도록 디자인된 제품입니다. 본 제품의 Enzyme Mix에는 chemically-modified hot start PCR 효소인 Hot-Taq polymerase와 함께 Thermo Reverse Transcriptase, 그리고 RNase Inhibitor가 포함 되어있으며, one-step RT-PCR용으로 최적화된 2x Reaction mix는 buffer, MgCl₂ 및 dNTP로 구성 되어있습니다. Carryover contaminant DNA에 의한 오염 방지를 위해 Heat Labile UDG와 dUTP가 적용된 제품을 선택할 수 있습니다.

Application

• Viral RNA detection
• Gene-expression analysis
• Molecular diagnosis
• Endpoint one-step RT-PCR from any RNA transcript

Figure 1. Multiplex one-step RT-PCR of up to 4 targets using HelixCript™ One-Step RT-PCR Kit [Hot-Taq].

Multiplex one-step RT-PCR in a single-tube reaction was performed with total RNA extracted from a hot pepper plant (Capsicum annuum) and by adding the indicated gene-specific primer sets. Each lane from 1 to 4 represents the progressive number of primer sets. The primer sets for the following genes were used; CaAPX1 (374 bp), CaActin (461 bp), CaPF1 (615 bp), and Cadhn (761 bp).

Properties: Conventional RT-PCR, Hot-start

Heat activation: +95 ℃ for 15 minutes after reverse transcription reaction

Activity test: Performance test in PCR (Human total RNA, Actin fragment): Corresponds to specification

Stability: 12 months at -20 ℃